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Thermo Scientific Lambda DNA HindIII Marker ready to use is recommended for sizing of linear double stranded large DNA on agarose gels Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme
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Image Search Results
Journal: BMC Genomics
Article Title: Pacbio sequencing of copper-tolerant Xanthomonas citri reveals presence of a chimeric plasmid structure and provides insights into reassortment and shuffling of transcription activator-like effectors among X. citri strains
doi: 10.1186/s12864-017-4408-9
Figure Lengend Snippet: Total genomic and plasmid extractions of strains were subjected to electrophoresis ( a ) and probed with pthA probe (using primer pthAF/R DIG as the probe), Xc-A306-p33 and Xc-A306-p64 plasmid specific probes by Southern hybridization ( b , c and d , respectively). Lanes 1 and 17 = DNA Molecular Weight Marker III DIG labeled; 2 = Erwinia stewartii SW2; 3 = Xc-A306 plasmid extraction (pXc-A306); 4 = Xc-A306 genomic DNA restricted with EcoRI (Xc-A306 EcoRI); 5 = pXc-03-1638-1-1; 6 = Xc-03-1638-1-1 EcoRI); 7 = pXc-A2090; 8 = Xc-A2090 EcoRI; 9 = pXc-A1660; 10 = Xc-A2090 EcoRI; 11 = pXc-AEtrog; 12 = Xc-AEtrog EcoRI; 13 = pXc-A100-Japan; 14 = Xc-A100 Japan EcoRI; 15 = pXc-A109 India; 16 = Xc-A109 India EcoRI. A = Ethidium bromide stained gel (agarose 0.7%). b Southern blot of gel in A with pthA probe. c Southern blot of gel in A with Xc-p33 probe (using primer SPCF33F/R DIG as the probe). d Southern blot of gel in A with Xc-p64 probe (using primer SPCF64F/R DIG as the probe)
Article Snippet: A
Techniques: Plasmid Preparation, Electrophoresis, Hybridization, Molecular Weight, Marker, Labeling, Extraction, Staining, Southern Blot